A Method Paper: Growth Effects of Marine and Freshwater Microalgae on Aquaculture Sludge Extracts

  • KASTURI ARUMUGAM University Selangor, Faculty of Engineering & Life Sciences, Department of Science & Biotechnology, 45600 Bestari Jaya, Selangor, Malaysia.
Keywords: Modified SE, extraction methods, microplate-incubation technique, microalgae

Abstract

Natural growth promoting nutrients from aquaculture sludge wastes can be used to maximize the microalgae growth rather than as pollutants that may degrade the ecosystem.

Purpose: This study identified the growth of  targeted microalgae in modififed growth medium containing Sabak Bernam shrimp pond and Kota Puteri fish pond sludge extracts.

Background: . Aquaculture industry is growing rapidly due to the increases in demand for fish and seafood. Apart from advancement, some concerns were also evoked such as degradation of ecosystem by the discharge of large volume of sludge to the environment. Aquaculture sludge not only having pollutants that degrade the ecosystem while have some nutrients that can enhance the growth of microalgae.

Design/Methodology/Approach: Conway or bold’s basal media (BBM) was supplemented with SE collected from Sabak Bernam shrimp pond (SB) and Kota Puteri fish pond (KP), and tested using a microplate-incubation technique. Five different extraction methods were carried out for both SE collected such as 105 °C, 105 °C twice, 121 °C, 121 °C twice and 24-hour natural extraction. Microalgae culture in the microplates containing control (media) and enriched (media + SE) samples were incubated for 9-days, at 25 °C with light intensity of 33.75 µmol photons m-2 s-1 at 12 hours’ light and dark cycle.

Results/Findings: The total dissolved nitrogen (TDN) and total dissolved phosphorus (TDP) of SB and KP SE on different extraction methods were calculated. The specific growth rate (SGR) and division rate of targeted microalgae on modified SE were calculated.

Conclusion and Implications: modified SE did influence the microalgae growth more than control. This study shows that modified SE could be used as medium for microalgae cultivation.

Published
2020-01-04
How to Cite
ARUMUGAM, K. (2020). A Method Paper: Growth Effects of Marine and Freshwater Microalgae on Aquaculture Sludge Extracts. Postgraduate Research Symposium - January 2020, 1(1). Retrieved from http://ojs.journals.unisel.edu.my/index.php/prsj20/article/view/26