A Data Paper: Decolourization of Congo Red by Rhodococcus sp. and its Optimization by Response Surface Methodology
Purpose: The study entails works related to bioremediation, especially textile wastewater containing dye by utilizing microorganism, particularly Rhodococcus bacteria to decolourize toxic and xenobiotic Congo red dye solution and degrade the dye compound.
Background: Textile industry is responsible directly or indirectly for environmental problem such as water pollution. However, conventional treatment methods have disadvantages, thus benefits of exploiting microorganisms for textile effluent treatment are actively studied by many researchers, similar to this study.
Design/Methodology/Approach: Laboratory work related to screening stage was started after discussion with supervisor. Twenty-three strains of Rhodococcus maintained at Unisel Culture Collection were revived from cryobeads stored at - 80 on nutrient agar (8 g/L) and streaked aseptically onto agar plates containing Congo red dye (0.0.2, 0.04 g/L) in duplicates. The plates were incubated at 30 and monitored for 7 days for growth.
Results/Findings: All 23 strains were capable of growing at both the concentrations. Some strains exhibited moderate growth whereas some strain exhibited good growth. This shows the bacteria has ability to grow on agar containing Congo red.
Conclusion and Implications: This shows Rhodococcus has the potential to tolerate toxic character of Congo red dye and possibly decolourize the dye. Further screening and optimization studies will be carried out in the upcoming semester to enhance the decolourization activity.